Staff member: Doctor Limei Chen (Professor )，Doctor HongJuan Nian（Associate Professor），Doctor Qi Chen（Lecturer）
Research Fields: In the field of plantC1 compound metabolic engineering, we study the metabolic mechanism of C1compound (methanol and formaldehyde) in plants, investigate the mechanismunderlying the effects of methanol stimulation and formaldehyde stress on plantgrowth at signal transduction level and develop transgenic plants with strongcapacity to remediate HCHO pollution by using novel genetic engineeringstrategy. In the field of stress plant molecular biology, we study themolecular mechanisms underlying the effects of acidic soil and Al stress,drought stress and obstacle factor stress of continuous cultivation undermodernized Agriculture conditions on plant growth, identify the stress-tolerantgenes in plants and develop effective methods to enhance plant growth undersuch stress conditions. In the field of stress micro-organism molecular biology,we identify the stress-tolerant genes in Al-tolerant yeasts and characterized CaM-binding proteinsfrom Al-tolerant yeasts.
Equipments: (1) Plant genetic engineering platform consists of Gene Pulser Xcell TMEletroporation System, clean bench, particle bombardment, Cyclone StoragePhosphor System Cyclone, HPLC, pH microelectrodes, Portable plant porometer,formaldehyde detector; (2) Micro-organismgenetic engineering platform includes Automatic Autoclav，constant temperature incubator, constanttemperature shaker, Sonicator, SpeedVac, Electrophoresis, -80ºC,-20ºC and 4 ºC Refrigerator, microscope；(3) Gene cloning platform consists of Common PCR instrument,Gradient PCR instrument, Gel imaging system, Constantincubator.
Research Projects: Subprojects of the National Basic Research Programme of China: (1) Genetic engineering on enhancement of southernalfalfa Al-resistance；(2) Cloning and application of Al-tolerant genesfrom legume grasses.The National Natural Science Foundation of China: (1)Installation of photosynthetic HCHO assimilation pathways in plants by geneticengineering;(2) The functional mechanism of C1 metabolic net in HCHO detoxification in Arabidopsisthanliana；(3) Characterization andfunctional analysis of CaM-binding proteins from Al-tolerant yeasts; (4) The molecular mechanism for Al-enhanced PM H+-ATPaseactivity and citrate secretion in Al-tolerant broad bean. Subproject ofScientific and Technology Research Programme of Yunnan: Investigation ofphysiological basis for wetland plants toclear agricultural wastewater; Subproject of The National Natural Science Foundation of China: Determinationof target protein expression level in transgenic plants and analysis ofmetabolism by ?xml:namespace>13C-NMR.
Research Collaboration: International Research Collaboration is conducted with Professor Izui in Schoolof Life Sicence of Kyoto University , Professor Katoin Agricultural School of Kyoto University in Japan to perform plant geneticengineering on augmentation of plant ability to assimilate HCHO and remediateHCHO pollution. National Research Collaboration isconducted with South-West University, Guangxi Academy of Science, Yunnantobacco Academy of Science, Yunnan Environmental Academy ofScience to study the molecular mechanism of plant resistance to Al and acidicsoil, physiologicalbasis for wetland plants to clearagricultural wastewater and thetechnology for determination of target protein expression level intransgenic plants and analysis of metabolism by 13C-NMR.
1. Overexpression of anHPS/PHI fusion enzyme from Mycobacterium gastri MB19 in chloroplasts of geranium enhances its ability toassimilate and phytoremediate HCHO. Biotechnology letter.2010.3
2. Overexpression ofmalate dehydrogenase in transgenic tobacco leaves: enhanced malate synthesisand augmented Al-resistance. Acta Physiol Plant.2010.5.
3. Transcription andphyologycal changes of alalfa in response to aluminium stress. Journal of Agricultural Science. 2011.11.
4. Construction andapplication of a Gateway entry vector with Rubisco small subunit promoter andits transit peptide sequence. Progress in Biochemistry andBiophysics. 2011.3.
5. Physiological andtranscriptional analysis of the effects of formaldehyde exposure on Arabidopsisthaliana. Acta Physiol Plant. 2011.11.
6. Simultaneousoverexpression of citrate synthesis and phosphoenolpyruvate carboxylase inleaves augments citrate exclusion and Al resistance in transgenic tobacco.Plant Mol. Biol Rep. 2012, 30:992-1005.
7. Overexpression ofdihydroxyacetate synthase and dihydroxyacetone kinase in chloroplasts install a novel photosynthetic HCHO-assimilation pathway intransgenic tobacco using modified Gateway entry vector. ActaPhysiol Plant. 2012. 3.
8. Changesin the Activity and Transcription of Antioxidant Enzymes in Response to AlStress in Black Soybeans. Plant Mol Biol Rep. 2012.5.
9. Effects offormaldehyde stress on physiological characteristics and gene expressionassociated with photosynthesis in Arabidopsis thaliana.Plant Mol Biol Rep.2012.30:1291-1302.
10. Phyiological andmolecular responses of broad bean (Vicia faba L.) to aluminium stress.Acta Physiol Plant. 2012.34:2251-2263.
11. Overexpression ofMsALMT1, from the aluminium-sensitive Meticago sativa, enhances malateexudation and aluminium resistance in tobacco. Plant Mol Biol Rep. 2012.12.
12. Physiological and transcriptionalanalysis of the effects of aluminium stress on Cryptococcus humicola.World J Microbiol Biotechnol.2012.28:2319-2329.
13. Isolation of Al-tolerant yeasts andidentification of their Al-tolerance characteristics. Journal of biological research-Thessaloniki.2012.6.
English book：Genetic Engineering of photosynthesisin C3 plants. Yunnan Science and TechnologyPress.